Dynamic transcriptome analysis measures rates of mRNA synthesis and decay in yeast
نویسندگان
چکیده
منابع مشابه
Dynamic transcriptome analysis measures rates of mRNA synthesis and decay in yeast
To obtain rates of mRNA synthesis and decay in yeast, we established dynamic transcriptome analysis (DTA). DTA combines non-perturbing metabolic RNA labeling with dynamic kinetic modeling. DTA reveals that most mRNA synthesis rates are around several transcripts per cell and cell cycle, and most mRNA half-lives range around a median of 11 min. DTA can monitor the cellular response to osmotic st...
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Standard transcriptomics measures total cellular RNA levels. Our understanding of gene regulation would be greatly improved if we could measure RNA synthesis and decay rates on a genome-wide level. To that end, the Dynamic Transcriptome Analysis (DTA) method has been developed. DTA combines metabolic RNA labeling with standard transcriptomics to measure RNA synthesis and decay rates in a precis...
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ژورنال
عنوان ژورنال: Molecular Systems Biology
سال: 2011
ISSN: 1744-4292,1744-4292
DOI: 10.1038/msb.2010.112